Theunissen P H, Leers M P, Bollen E C
Department of Pathology, De Wever Hospital, Heerlen, The Netherlands.
Histopathology. 1992 Mar;20(3):251-5. doi: 10.1111/j.1365-2559.1992.tb00964.x.
An immunohistochemical study of non-small cell lung carcinoma using PC10, a monoclonal antibody against PCNA, was performed on tissues routinely processed with formalin fixation and paraffin embedding. The PCNA labelling index and mitotic index were determined from sections of these tissues. Tumours showed a high mean PCNA labelling index of 53.3%. The mean mitotic index was 10.3/1000 cells. Inter-examiner agreement of mitotic counting was good. A linear correlation between the PCNA labelling index and mitotic index was demonstrated (r = 0.71, P less than 0.00001). It is concluded that immunohistochemical nuclear labelling with anti-PCNA on routinely processed tissue is a simple technique for the assessment of proliferation in non-small cell lung carcinoma.
利用抗增殖细胞核抗原（PCNA）的单克隆抗体PC10，对经福尔马林固定和石蜡包埋的常规处理组织进行非小细胞肺癌的免疫组织化学研究。从这些组织切片中确定PCNA标记指数和有丝分裂指数。肿瘤的平均PCNA标记指数较高，为53.3%。平均有丝分裂指数为10.3/1000个细胞。有丝分裂计数的检查者间一致性良好。PCNA标记指数与有丝分裂指数之间呈线性相关（r = 0.71，P<0.00001）。结论是，对常规处理组织进行抗PCNA免疫组织化学细胞核标记是评估非小细胞肺癌增殖的一种简单技术。
