Le Moine C, Young W S
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3285-9. doi: 10.1073/pnas.89.8.3285.
Gene expression within the central nervous system is regulated by complex interactions of DNA-binding proteins, among which are the POU domain-containing proteins, which are distantly related to homeobox proteins. These POU domain-containing proteins have been implicated in control of transcription and replication within the central nervous system. We used degenerate primers with the PCR to isolate another POU domain-containing cDNA, RHS2, from hypothalamic RNA. Isolation of a putative full-length cDNA was accomplished by using serial dilutions of a hypothalamic cDNA library grown on solid medium. This member of the class III POU family is expressed in rats from embryonic day 11.5 into adulthood, being especially prominent in the brain. We performed double-labeling hybridization histochemistry and determined that RHS2 is coexpressed with a variety of neuropeptides in medium-sized neurons in the caudate putamen and with dynorphin in the paraventricular and supraoptic nuclei of the hypothalamus. Expression of RHS2 in the caudate putamen was increased by elimination of its nigrostriatal dopaminergic innervation.
中枢神经系统内的基因表达受DNA结合蛋白复杂相互作用的调控，其中含POU结构域的蛋白与同源框蛋白有较远的亲缘关系。这些含POU结构域的蛋白与中枢神经系统内的转录和复制调控有关。我们使用简并引物通过聚合酶链反应（PCR）从下丘脑RNA中分离出另一个含POU结构域的cDNA，即RHS2。通过对在固体培养基上生长的下丘脑cDNA文库进行系列稀释，获得了一个假定的全长cDNA。这个III类POU家族成员在大鼠胚胎第11.5天到成年期均有表达，在脑中尤为突出。我们进行了双标记杂交组织化学分析，确定RHS2在尾状壳核的中型神经元中与多种神经肽共表达，在下丘脑室旁核和视上核中与强啡肽共表达。通过消除其黑质纹状体多巴胺能神经支配，尾状壳核中RHS2的表达增加。
