Hoch M, Gerwin N, Taubert H, Jäckle H
Max-Planck-Institut für Biophysikalische Chemie, Abteilung Molekulare Entwicklungsbiologie, Göttingen, Federal Republic of Germany.
Science. 1992 Apr 3;256(5053):94-7. doi: 10.1126/science.1348871.
A 730-base pair element regulates expression of the Drosophila gap gene Krüppel (Kr) in response to the fly anterior morphogen bicoid (bcd). Two hormone receptor-like proteins, encoded by the genes knirps (kni) and tailless (tll), bind specifically to the element. In vitro, kni protein competes with the homeodomain-containing bcd protein in binding to a 16-base pair target sequence. In vivo experiments suggest that both kni and tll act as competitive repressors of bcd-mediated activation of Kr. These results suggest a mechanism by which developmental genes can be regulated in response to an activating morphogen gradient antagonized by repressors.
一个730个碱基对的元件可响应果蝇前部形态发生素双胸（bcd）来调节果蝇间隙基因克虏伯氏（Kr）的表达。由无尾（kni）基因和无尾（tll）基因编码的两种激素受体样蛋白可特异性结合该元件。在体外，kni蛋白与含同源结构域的bcd蛋白竞争结合一个16个碱基对的靶序列。体内实验表明，kni和tll均作为bcd介导的Kr激活的竞争性抑制剂。这些结果提示了一种机制，通过该机制发育基因可响应由抑制剂拮抗的激活形态发生素梯度进行调节。
