Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Cidade Universitária, Av. Prof. Lineu Prestes, 748 São Paulo, SP, Brazil 05508-000.
J Bioenerg Biomembr. 2011 Oct;43(5):483-91. doi: 10.1007/s10863-011-9377-0. Epub 2011 Jul 21.
We studied the importance of respiratory fitness in S. cerevisiae lifespan, response to caloric restriction (CR) and mtDNA stability. Mutants harboring mtDNA instability and electron transport defects do not respond to CR, while tricarboxylic acid cycle mutants presented extended lifespans due to CR. Interestingly, mtDNA is unstable in cells lacking dihydrolipoyl dehydrogenase under CR conditions, and cells lacking aconitase under standard conditions (both enzymes are components of the TCA and mitochondrial nucleoid). Altogether, our data indicate that respiratory integrity is required for lifespan extension by CR and that mtDNA stability is regulated by nucleoid proteins in a glucose-sensitive manner.
我们研究了呼吸健身在酿酒酵母寿命、对热量限制（CR）的反应和 mtDNA 稳定性中的重要性。携带 mtDNA 不稳定性和电子传递缺陷的突变体对 CR 没有反应，而三羧酸循环突变体由于 CR 而表现出延长的寿命。有趣的是，在 CR 条件下缺乏二氢脂酰脱氢酶的细胞中的 mtDNA 不稳定，而在标准条件下缺乏顺乌头酸酶的细胞中的 mtDNA 不稳定（这两种酶都是 TCA 和线粒体核小体的组成部分）。总的来说，我们的数据表明，呼吸完整性是 CR 延长寿命所必需的，并且 mtDNA 稳定性受核小体蛋白以葡萄糖敏感的方式调控。
