Hayashi M, Yamashita A, Shimizu K, Sogawa K, Fujii Y
Department of Physiology, Kyoto University, Aichi, Japan.
Brain Res Dev Brain Res. 1990 Dec 1;57(1):37-41. doi: 10.1016/0165-3806(90)90182-x.
Somatostatin (SRIF) mRNA was determined in the developing monkey frontal and cerebellar cortices by the dot blot and the northern blot analyses at embryonic day 120 (E120), embryonic day 140 (E140), newborn stage (Nb), postnatal day 60 (P60) and adult stage (Ad.) At E120, at which time the migration of the cortical neurons had already been completed, SRIF mRNA was detectable with 50% of the maximal value at E140 in the cerebral frontal cortex (von Bonin and Bailey's area FD). After E140, the level of mRNA gradually declined to the adult level by P60 with 25% of the maximal value. In the cerebellum, SRIF mRNA was highly expressed at E120. The level decreased to 18% of the maximum at E140. Between the newborn and adult stages, there existed no positive signal of the mRNA. In contrast, both fetal and adult liver tissues contained no amounts of SRIF mRNA. We discussed the physiological meanings of the enhanced SRIF gene expression in the developing monkey cerebral and cerebellar cortices.
通过斑点印迹法和Northern印迹法，在胚胎第120天（E120）、胚胎第140天（E140）、新生期（Nb）、出生后第60天（P60）和成年期（Ad.），对发育中的猕猴额叶和小脑皮质中的生长抑素（SRIF）mRNA进行了测定。在E120时，此时皮质神经元的迁移已经完成，在大脑额叶皮质（冯·博宁和贝利氏FD区）中可检测到SRIF mRNA，其水平为E140时最大值的50%。E140之后，mRNA水平逐渐下降，到P60时降至成年水平，为最大值的25%。在小脑中，SRIF mRNA在E120时高度表达。该水平在E140时降至最大值的18%。在新生期和成年期之间，未检测到mRNA的阳性信号。相比之下，胎儿和成年肝脏组织中均未检测到SRIF mRNA。我们讨论了发育中的猕猴大脑和小脑皮质中SRIF基因表达增强的生理意义。
