Reuke B, Korn S, Eisenreich W, Bacher A
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Garching, Germany.
J Bacteriol. 1992 Jun;174(12):4042-9. doi: 10.1128/jb.174.12.4042-4049.1992.
The incorporation of 13C- and 14C-labeled precursors into 5-deaza-7,8-didemethyl-8-hydroxyriboflavin (factor F0) was studied with growing cells of Methanobacterium thermoautotrophicum. 5-Amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione was incorporated into the deazaflavin and into riboflavin without dilution. Tyrosine and 4-hydroxyphenylpyruvate were incorporated into the deazaflavin and into cellular protein. 4-Hydroxybenzaldehyde was not incorporated. A reaction mechanism is proposed for the formation of the deazaflavin chromophore from 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione and tyrosine or 4-hydroxyphenylpyruvate.
利用嗜热自养甲烷杆菌的生长细胞，研究了将13C和14C标记的前体掺入5-脱氮-7,8-二去甲基-8-羟基核黄素（因子F0）的情况。5-氨基-6-核糖基氨基-2,4(1H,3H)-嘧啶二酮未被稀释地掺入脱氮黄素和核黄素中。酪氨酸和4-羟基苯丙酮酸被掺入脱氮黄素和细胞蛋白中。4-羟基苯甲醛未被掺入。提出了一种由5-氨基-6-核糖基氨基-2,4(1H,3H)-嘧啶二酮与酪氨酸或4-羟基苯丙酮酸形成脱氮黄素发色团的反应机制。
