枯草芽孢杆菌中核黄素的生物合成：四碳部分的来源。
Biosynthesis of riboflavin in Bacillus subtilis: origin of the four-carbon moiety.
作者信息
Le Van Q, Keller P J, Bown D H, Floss H G, Bacher A
出版信息
J Bacteriol. 1985 Jun;162(3):1280-4. doi: 10.1128/jb.162.3.1280-1284.1985.
Abstract
We studied the incorporation of [1-13C]ribose and [1,3-13C2]glycerol into the riboflavin precursor 6,7-dimethyl-8-ribityllumazine, using a riboflavin-deficient mutant of Bacillus subtilis. The formation of the pyrazine ring requires the addition of a four-carbon moiety to a pyrimidine precursor. The results show that C-6 alpha, C-6, C-7, and C-7 alpha of 6,7-dimethyl-8-ribityllumazine were biosynthetically equivalent to C-1, C-2, C-3, and C-5 of a pentose phosphate. C-4 of the pentose precursor was lost through an intramolecular skeletal rearrangement. Thus, the last steps in the biosynthesis of 6,7-dimethyl-8-ribityllumazine apparently involve the same mechanism in bacteria as in fungi.
摘要
我们使用枯草芽孢杆菌的核黄素缺陷型突变体，研究了[1-¹³C]核糖和[1,3-¹³C₂]甘油掺入核黄素前体6,7-二甲基-8-核糖基卢马嗪的情况。吡嗪环的形成需要在嘧啶前体上添加一个四碳部分。结果表明，6,7-二甲基-8-核糖基卢马嗪的C-6α、C-6、C-7和C-7α在生物合成上等同于磷酸戊糖的C-1、C-2、C-3和C-5。戊糖前体的C-4通过分子内骨架重排而丢失。因此，6,7-二甲基-8-核糖基卢马嗪生物合成的最后步骤在细菌中显然与在真菌中涉及相同的机制。
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