Ding S F, Habib N A, Delhanty J D, Bowles L, Greco L, Wood C, Williamson R C, Dooley J S
University Department of Surgery, Royal Free Hospital School of Medicine, London, UK.
Br J Cancer. 1992 Jun;65(6):809-12. doi: 10.1038/bjc.1992.173.
Little is known of the molecular-genetic changes in carcinoma of the pancreas (CaP). In order to investigate the allele loss, or loss of heterozygosity (LOH), in CaP, we studied 13 patients with exocrine CaP and two with endocrine CaP using restriction fragment length polymorphism analysis. Twenty probes assigned to chromosomes 1, 5, 7, 9, 11, 12, 13, 14, 16, 17 and 18 were used. The frequency of LOH, or fractional allele loss (FAL), was found in two endocrine tumours to be 0.333 and 0.455 respectively; and FAL in 13 oxocrine tumours ranged from 0 to 0.25. Allele loss was shown in both exocrine and endocrine tumours by the probes Lambda MS1 at 1p33-35, and pMS51 at 11q13. Probes for other chromosomes have as yet shown no consistent LOH. In conclusion, the study showed LOH on chromosomes 1 and 11 in both exocrine and endocrine CaP.
关于胰腺癌（CaP）的分子遗传学改变，人们了解甚少。为了研究CaP中的等位基因缺失或杂合性缺失（LOH），我们使用限制性片段长度多态性分析对13例外分泌性CaP患者和2例内分泌性CaP患者进行了研究。使用了分配到1、5、7、9、11、12、13、14、16、17和18号染色体的20个探针。发现两个内分泌肿瘤中的LOH频率或等位基因缺失分数（FAL）分别为0.333和0.455；13个外分泌肿瘤中的FAL范围为0至0.25。位于1p33 - 35的探针Lambda MS1和位于11q13的探针pMS51在外分泌和内分泌肿瘤中均显示出等位基因缺失。其他染色体的探针尚未显示出一致的LOH。总之，该研究表明外分泌性和内分泌性CaP在1号和11号染色体上均存在LOH。
