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表达CD45RA的人CD4+T细胞在体外激活后可获得CD45RO+辅助性T细胞的淋巴因子基因表达。

Human CD4+ T cells expressing CD45RA acquire the lymphokine gene expression of CD45RO+ T-helper cells after activation in vitro.

作者信息

Kristensson K, Borrebaeck C A, Carlsson R

机构信息

BioInvent International AB, Lund, Sweden.

出版信息

Immunology. 1992 May;76(1):103-9.

PMID:1352764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1421743/
Abstract

CD4+ T cells were separated into subpopulations according to their expression of different isoforms of the CD45R molecule, i.e. CD45RA and CD45RO. The separated cells were activated with staphylococcal enterotoxin A (SEA) in the presence of formalin fixed Raji cells. Each set of cells was activated twice with a 6-day interval, and the lymphokine gene expression during the first 3 days after initiation of each stimulation was followed by use of polymerase chain reaction (PCR) technology. The lymphokine messenger RNA (mRNA) profiles were found to differ between the subsets, since after the first stimulation the CD45RA+ cells produced mRNA encoding interleukin-2 (IL-2) and IL-1 alpha, whereas the CD45RO+ cells transcribed genes for IL-1 alpha, IL-2, IL-4, IL-5 and interferon-gamma (IFN-gamma). After 6 days of SEA stimulation both populations were mainly CD45RO reactive, and when restimulated displayed the lymphokine mRNA profile restricted to this subset. These results indicate that the CD45RA subset is a precursor of the CD45RO and further strengthen the hypothesis that the former cell population represents naive whereas the latter subset represents memory T cells within the CD4 subset.

摘要

CD4 + T细胞根据其CD45R分子不同异构体（即CD45RA和CD45RO）的表达被分离成亚群。分离出的细胞在甲醛固定的Raji细胞存在的情况下用葡萄球菌肠毒素A（SEA）激活。每组细胞以6天的间隔激活两次，并且在每次刺激开始后的前3天期间，通过使用聚合酶链反应（PCR）技术追踪淋巴因子基因表达。发现亚群之间的淋巴因子信使核糖核酸（mRNA）谱不同，因为在第一次刺激后，CD45RA +细胞产生编码白细胞介素-2（IL-2）和IL-1α的mRNA，而CD45RO +细胞转录IL-1α、IL-2、IL-4、IL-5和干扰素-γ（IFN-γ）的基因。SEA刺激6天后，两个群体主要对CD45RO有反应，并且在再次刺激时显示出仅限于该亚群的淋巴因子mRNA谱。这些结果表明，CD45RA亚群是CD45RO的前体，并进一步强化了以下假设：前一个细胞群体代表幼稚细胞，而后者亚群代表CD4亚群内的记忆T细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/1f4c78815b0c/immunology00104-0109-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/b3a00d3f0d2f/immunology00104-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/203f0d84030e/immunology00104-0108-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/728b6d205678/immunology00104-0108-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/c1b30aedf7d5/immunology00104-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/1f4c78815b0c/immunology00104-0109-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/b3a00d3f0d2f/immunology00104-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/203f0d84030e/immunology00104-0108-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/728b6d205678/immunology00104-0108-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/c1b30aedf7d5/immunology00104-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dc1/1421743/1f4c78815b0c/immunology00104-0109-b.jpg

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本文引用的文献

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Staphylococcal protein A (SpA) does not induce production of interferon-gamma in human mononuclear blood cells.葡萄球菌蛋白A（SpA）不会诱导人单核血细胞产生γ干扰素。
Cell Immunol. 1984 Jun;86(1):136-44. doi: 10.1016/0008-8749(84)90366-6.
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T cell clone producing an IL 1-like activity after stimulation by antigen-presenting B cells.在抗原呈递B细胞刺激后产生白细胞介素-1样活性的T细胞克隆。
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Binding of staphylococcal enterotoxin A to accessory cells is a requirement for its ability to activate human T cells.
诱导性 Sézary 综合征 PBMCs 表达免疫应答基因的能力较差，这些基因在受刺激的记忆 T 细胞中上调。
J Dermatol Sci. 2010 Oct;60(1):8-20. doi: 10.1016/j.jdermsci.2010.07.007. Epub 2010 Jul 22.
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CD45RO enriches for activated, highly mutated human germinal center B cells.CD45RO可富集活化的、高度突变的人类生发中心B细胞。
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Th1 cytokine patterns in cervical human papillomavirus infection.宫颈人乳头瘤病毒感染中的1型辅助性T细胞细胞因子模式
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CD2-mediated stimulation of the naive CD4+ T-cell subset promotes the development of skin-associated cutaneous lymphocyte antigen-positive memory cells.CD2介导的初始CD4 + T细胞亚群刺激促进皮肤相关皮肤淋巴细胞抗原阳性记忆细胞的发育。
Immunology. 1996 Jun;88(2):207-13. doi: 10.1111/j.1365-2567.1996.tb00006.x.
8
Interleukin 2 induces the expression of CD45RO and the memory phenotype by CD45RA+ peripheral blood lymphocytes.白细胞介素2可诱导CD45RA⁺外周血淋巴细胞表达CD45RO并呈现记忆表型。
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The isolation and characterization of the human suppressor inducer T cell subset.人类抑制诱导性T细胞亚群的分离与鉴定
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Eur J Immunol. 1987 Jan;17(1):97-103. doi: 10.1002/eji.1830170117.