Wickes B L, Hicks J B, Merz W G, Kwon-Chung K J
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
J Gen Microbiol. 1992 May;138(5):901-7. doi: 10.1099/00221287-138-5-901.
Three sets of medically important yeasts, Candida albicans, C. tropicalis, and C. krusei, were compared with their putative synonyms (C. langeronii and C. claussenii, C. paratropicalis, and Itssatchenkia orientalis, respectively) to determine if these synonyms are genetically distinguishable from each other. Pulsed-field electrophoresis and hybridization to species-specific probes were used to accomplish this goal. The species-specific probes for C. albicans and C. tropicalis have been previously described (27A and CT13.8, respectively) whereas the probe for C. krusei (CK3) was cloned in this study. No distinguishing characteristics between synonyms were identified, thus supporting the current taxonomic treatment of these organisms.
将三组具有医学重要性的酵母，白色念珠菌、热带念珠菌和克鲁斯念珠菌，与其假定的同义词（分别为兰氏念珠菌和克劳森念珠菌、副热带念珠菌和东方伊萨酵母）进行比较，以确定这些同义词在基因上是否彼此可区分。采用脉冲场电泳和与物种特异性探针杂交来实现这一目标。白色念珠菌和热带念珠菌的物种特异性探针先前已有描述（分别为27A和CT13.8），而克鲁斯念珠菌的探针（CK3）是在本研究中克隆的。未发现同义词之间的区别特征，因此支持对这些生物体目前的分类处理。
