Mizuki N, Inoko H, Sugimura K, Nishimura K, Nakamura S, Tanaka H, Mizuki N, Mizuki H, Inaba G, Tsuji K
Department of Ophthalmology, Yokohama City University School of Medicine, Japan.
Invest Ophthalmol Vis Sci. 1992 Oct;33(11):3084-90.
Behçet's disease is known to be associated with HLA-B51. To address the possibility that a non-human leukocyte antigen (HLA) gene closely linked to the HLA-B gene, such as tumor necrosis factor (TNF)-alpha, TNF-beta, or ECl (the locus that determines the susceptibility to alloreactive natural killer [NK] cells), is involved in the susceptibility to Behçet's disease, NcoI and EcoRI restriction fragment length polymorphisms in the TNF-beta gene and the susceptibility to lysis by alloreactive NK cells were investigated in Behçet's patients. In our NcoI restriction fragment length polymorphism (RFLP) analysis in the TNF-beta gene, the frequency of the NcoI 5.5 kb homozygote was decreased considerably in the patients, especially those with the ocular lesions, in relation to the healthy controls. However, no significant difference was observed between these groups in the EcoRI RFLP band distribution in this gene or the in susceptibility to lysis by alloreactive NK cells. These results indicated that a non-HLA gene located around the TNF gene region centromic of the HLA-B gene was a candidate to control the genetic susceptibility to Behçet's disease.
已知白塞病与HLA - B51相关。为了探究与HLA - B基因紧密连锁的非人类白细胞抗原（HLA）基因，如肿瘤坏死因子（TNF）-α、TNF -β或ECl（决定同种异体反应性自然杀伤[NK]细胞易感性的基因座）是否参与白塞病的易感性，对白塞病患者的TNF -β基因中的NcoI和EcoRI限制性片段长度多态性以及同种异体反应性NK细胞的裂解易感性进行了研究。在我们对TNF -β基因的NcoI限制性片段长度多态性（RFLP）分析中，与健康对照相比，患者中NcoI 5.5 kb纯合子的频率显著降低，尤其是有眼部病变的患者。然而，在该基因的EcoRI RFLP条带分布或同种异体反应性NK细胞的裂解易感性方面，这些组之间未观察到显著差异。这些结果表明，位于HLA - B基因着丝粒侧TNF基因区域附近的一个非HLA基因是控制白塞病遗传易感性的一个候选基因。
