Sharp F, Hay J B, Hodgins M B
J Endocrinol. 1976 Sep;70(3):491-9. doi: 10.1677/joe.0.0700491.
Fresh scalp, genital, chest and axillary skin from human foetuses of 12-41 weeks' maturity was incubated in Krebs improved Ringer I medium with (7alpha-3h)dehydroepiandrosterone, (7alpha-3H)testosterone and (7alpha-3H)androstenedione. The metabolites identified were androstenedione, 5alpha-androstane-3alpha, 17beta-diol, 5alpha-androstane-3beta, 17beta-diol, 5-androstene-3beta, 17beta-diol and testosterone. The results provide evidence for the presence of 3beta-hydroxysteroid dehydrogenase, delta4-5 isomerase, 17beta-hydroxysteroid dehydrogenase, delta4-3-oxosteroid-5alpha reductase and 3alpha-hydroxysteroid dehydrogenase in human foetal skin. There were quantitative differences in the various enzyme activities between different body sites and skin specimens of different gestational age. 5alpha-Reductase activity was particularly high in genital skin. 3beta-Hydroxysteroid dehydrogenase delta4-5 isomerase activity was low in skin from a 12-week foetus, but high in skin specimens from 28-, 38- and 41-week foetuses. 17beta-Hydroxysteroid dehydrogenase activity was already high in the skin of the 12-week foetus and remained so in the older foetuses. These results were correlated with the development of the foetal sebaceous glands, and were in general agreement with a parallel enzyme histochemical study. The role of androgen metabolism in human foetal skin is discussed.
将12至41周龄成熟度的人类胎儿的新鲜头皮、生殖器、胸部和腋窝皮肤，置于含有（7α-³H）脱氢表雄酮、（7α-³H）睾酮和（7α-³H）雄烯二酮的改良克雷布斯林格I培养基中进行孵育。鉴定出的代谢产物有雄烯二酮、5α-雄甾烷-3α,17β-二醇、5α-雄甾烷-3β,17β-二醇、5-雄烯-3β,17β-二醇和睾酮。这些结果为人类胎儿皮肤中存在3β-羟基类固醇脱氢酶、Δ⁴-⁵异构酶、17β-羟基类固醇脱氢酶、Δ⁴-3-氧代类固醇-5α还原酶和3α-羟基类固醇脱氢酶提供了证据。不同身体部位以及不同胎龄的皮肤标本之间，各种酶活性存在定量差异。5α-还原酶活性在生殖器皮肤中特别高。3β-羟基类固醇脱氢酶Δ⁴-⁵异构酶活性在12周龄胎儿的皮肤中较低，但在28周、38周和41周龄胎儿的皮肤标本中较高。17β-羟基类固醇脱氢酶活性在12周龄胎儿的皮肤中就已经很高，在较大胎儿的皮肤中依然如此。这些结果与胎儿皮脂腺的发育相关，并且总体上与一项平行的酶组织化学研究结果一致。文中讨论了雄激素代谢在人类胎儿皮肤中的作用。
