Hay J B, Hodgins M B
J Endocrinol. 1978 Oct;79(1):29-39. doi: 10.1677/joe.0.0790029.
The distribution of androgen metabolism in human skin was studied using tissues isolated either by direct dissection of axillary skin or by dissection of collagenase-digested forehead and axillary skin. All tissues (epidermis, sweat glands, sebaceous glands, hair follicles and dermis) were found to contain 17beta-, 3beta- and 3alpha-hydroxysteroid dehydrogenase (HSD) activities, 3beta-hydroxysteroid dehydrogenase-delta4--5 isomerase (delta5-3beta-HSD) activity and 5alpha-reductase activity. All tissues converted testosterone into 5alpha-dihydrotestosterone. In confirmation of previous histochemical studies, over 90% of the delta5-3beta-HSD of forehead skin was found in the sebaceous glands. In forehead skin, 40--66% of the 5alpha-reductase activity was in the sebaceous glands, while in axillary skin 50--70% was in the sweat glands, especially the apocrine glands. There was a more even distribution of 17beta-HSD activity in skin tissues than histochemical studies have indicated previously. Knowledge of the distribution of these enzymes has helped in the understanding of the function of androgen metabolism in skin.
利用直接解剖腋窝皮肤或解剖经胶原酶消化的前额和腋窝皮肤所分离出的组织，对雄激素在人体皮肤中的代谢分布进行了研究。结果发现，所有组织（表皮、汗腺、皮脂腺、毛囊和真皮）均含有17β-、3β-和3α-羟基类固醇脱氢酶（HSD）活性、3β-羟基类固醇脱氢酶-δ4-5异构酶（δ5-3β-HSD）活性和5α-还原酶活性。所有组织均能将睾酮转化为5α-二氢睾酮。为证实先前的组织化学研究结果，发现前额皮肤中超过90%的δ5-3β-HSD存在于皮脂腺中。在前额皮肤中，5α-还原酶活性的40%-66%存在于皮脂腺中，而在腋窝皮肤中，50%-70%存在于汗腺，尤其是顶泌汗腺中。皮肤组织中17β-HSD活性的分布比先前组织化学研究所表明的更为均匀。了解这些酶的分布有助于理解雄激素在皮肤中的代谢功能。
