Jia H G, Rao Z R, Shi J W
Department of Anatomy, Fourth Military Medical University, Shaanxi, People's Republic of China.
Brain Res. 1992 Aug 28;589(1):167-70. doi: 10.1016/0006-8993(92)91180-m.
By using a double-labeling produce of retrograde horseradish peroxidase (HRP) tracing and the immunocytochemical localization of tyrosine hydroxylase (TH), the present study ascertained that the axonal fibers of catecholaminergic neurons in the caudal ventrolateral medulla projected to the central amygdaloid nucleus in the rat. The majority of double-labeled cells were observed primarily around the level of the obex. 92% of HRP retrogradely labeled cells contained TH-like immunoreactive (TH-IR), but only 31% of TH-IR cells was labeled with HRP.
通过使用逆行辣根过氧化物酶(HRP)追踪的双标记产物和酪氨酸羟化酶(TH)的免疫细胞化学定位,本研究确定大鼠延髓尾端腹外侧的儿茶酚胺能神经元的轴突纤维投射到中央杏仁核。大多数双标记细胞主要在闩平面水平周围被观察到。92%的HRP逆行标记细胞含有TH样免疫反应性(TH-IR),但仅31%的TH-IR细胞被HRP标记。