Takahashi M, Yokota H, Ayusawa D, Oishi M, Kunieda T
Institute of Applied Microbiology, University of Tokyo, Japan.
Biochem Genet. 1992 Oct;30(9-10):537-44. doi: 10.1007/BF01037592.
A novel restriction fragment length polymorphism (RFLP) in inbred rats was revealed by Southern blot analysis with a clone arbitrarily chosen from a rat genomic library as a probe. A clone named alpha 403 showed interstrain variations in the length of the EcoRI and HindIII fragments. The EcoRI fragments were either 0.7 or 3 kb, those of HindIII were either 4.5 or 5 kb, and three types were identified as combinations of those fragments in 20 inbred rat strains. These types segregated in backcross progeny as codominant alleles. The locus for the RFLP was thus named A403. Analysis of linkage between the RFLP locus and 13 other loci reveal that the A403 locus was closely linked to the Cs-1 locus (15 +/- 5.2%), which belongs to rat linkage group XIII.
用从大鼠基因组文库中随机挑选的一个克隆作为探针，通过Southern印迹分析，在近交系大鼠中发现了一种新的限制性片段长度多态性（RFLP）。一个名为α403的克隆在EcoRI和HindIII片段长度上表现出品系间差异。EcoRI片段为0.7 kb或3 kb，HindIII片段为4.5 kb或5 kb，在20个近交大鼠品系中，这两种片段的组合可鉴定出三种类型。这些类型在回交后代中作为共显性等位基因分离。因此，该RFLP的基因座被命名为A403。对该RFLP基因座与其他13个基因座之间的连锁分析表明，A403基因座与Cs-1基因座紧密连锁（15±5.2%），Cs-1基因座属于大鼠连锁群XIII。
