Prince H E, Jensen E R, York J
Cellular Immunology Laboratory, American Red Cross Blood Services, Southern California Region, Los Angeles 90006.
Clin Immunol Immunopathol. 1992 Dec;65(3):201-6. doi: 10.1016/0090-1229(92)90147-g.
Previous studies showed that over 70% of HTLV-seropositive blood donors from the Los Angeles area are infected with HTLV-II; further, mononuclear cells from about half of these HTLV-II+ donors exhibit spontaneous lymphocyte proliferation (SLP) during in vitro culture. To determine if HTLV-II+SLP+ donors exhibit more marked immune system changes than HTLV-II+SLP- donors, lymphocyte subsets for these two HTLV-II+ groups were compared to an uninfected control group. The percentage of lymphocytes expressing CD3 was significantly increased and the percentage expressing a CD16/56+CD3- phenotype (natural killer cells) was significantly decreased in the HTLV-II+SLP+ group (N = 34) versus the control group (N = 49). On the basis of absolute numbers, the lymphocyte number was significantly higher in the HTLV-II+SLP+ group than in the control group and reflected significant increases in the numbers of both CD4 and CD8 subsets of T cells. Analysis of proportional changes in CD4 and CD8 cell subsets revealed significant increases in the proportions of CD4 cells expressing HLA-DR, CD8 cells expressing HLA-DR, and CD8 cells expressing CD45RO for the HTLV-II+SLP+ group versus the control group. For all phenotypic parameters measured, no significant differences were noted when comparing the HTLV-II+SLP- group (N = 21) and the control group. Cell culture experiments utilizing purified CD4 cells and CD8 cells from a subset of each study group revealed that in vitro spontaneous proliferative capacity resides within both the CD4 cell and CD8 cell populations from SLP+ individuals. These findings show that changes in circulating lymphocyte subsets in HTLV-II infection are found only in association with SLP, and that the capacity to exhibit SLP characterizes both CD4 and CD8 lymphocyte subsets.
先前的研究表明，洛杉矶地区超过70%的HTLV血清反应阳性献血者感染了HTLV-II；此外，这些HTLV-II阳性献血者中约一半的单核细胞在体外培养期间表现出自发性淋巴细胞增殖（SLP）。为了确定HTLV-II+SLP+献血者是否比HTLV-II+SLP-献血者表现出更明显的免疫系统变化，将这两个HTLV-II阳性组的淋巴细胞亚群与未感染的对照组进行了比较。与对照组（N = 49）相比，HTLV-II+SLP+组（N = 34）中表达CD3的淋巴细胞百分比显著增加，而表达CD16/56+CD3-表型（自然杀伤细胞）的百分比显著降低。基于绝对数量，HTLV-II+SLP+组中的淋巴细胞数量显著高于对照组，并且反映出T细胞的CD4和CD8亚群数量均显著增加。对CD4和CD8细胞亚群比例变化的分析显示，与对照组相比，HTLV-II+SLP+组中表达HLA-DR的CD4细胞、表达HLA-DR的CD8细胞以及表达CD45RO的CD8细胞的比例显著增加。对于所有测量的表型参数，在比较HTLV-II+SLP-组（N = 21）和对照组时未发现显著差异。利用每个研究组的一个亚组的纯化CD4细胞和CD8细胞进行的细胞培养实验表明，体外自发增殖能力存在于SLP+个体的CD4细胞和CD8细胞群体中。这些发现表明，HTLV-II感染中循环淋巴细胞亚群的变化仅与SLP相关，并且表现出SLP的能力是CD4和CD8淋巴细胞亚群的特征。
