DEICHER H R, HOLMAN H R, KUNKEL H G
J Exp Med. 1959 Jan 1;109(1):97-114. doi: 10.1084/jem.109.1.97.
The sera of certain patients with systemic lupus erythematosus contain an antibody-like substance capable of reacting with highly purified DNA preparations from widely divergent sources. Precipitin reactions have been demonstrated by double diffusion in agar and quantitative precipitin curves have been obtained. Complement was observed to be fixed in the reaction. Evidence was obtained that the serum factor possessed antigenic properties similar to those of gamma-globulins and migrated with this fraction on zone electrophoresis. The interaction of this factor with DNA exhibited certain specific characteristics which differ considerably from non-specific reactions between DNA and proteins in general. The DNA-precipitating factor appeared to be one of a number of related factors reacting with nuclear constituents of many different cells. It differed in certain respects from the "LE factor" which is responsible for the formation of "LE cells." The accumulated evidence, although not yet conclusive, favors the concept that the precipitating factor represents an antibody to DNA, and that it is one of a number of autoantibodies elicited in this disease.
某些系统性红斑狼疮患者的血清中含有一种抗体样物质，它能够与来自广泛不同来源的高度纯化的DNA制剂发生反应。通过琼脂双向扩散已证实了沉淀反应，并获得了定量沉淀曲线。观察到补体在反应中被固定。有证据表明，血清因子具有与γ-球蛋白相似的抗原特性，并在区带电泳中与该部分一起迁移。该因子与DNA的相互作用表现出某些特定特征，这些特征与一般情况下DNA和蛋白质之间的非特异性反应有很大不同。DNA沉淀因子似乎是与许多不同细胞的核成分发生反应的多种相关因子之一。它在某些方面与负责形成“LE细胞”的“LE因子”不同。尽管证据尚不确凿，但积累的证据支持这样一种观点，即沉淀因子代表DNA抗体，并且它是这种疾病中引发的多种自身抗体之一。
