Gupta A K, Lad V J, Ghosh S N
National Institute of Virology, Pune, India.
Acta Virol. 1993 Feb;37(1):93-6.
A modified immunofluorescent method employing anti-Japanese encephalitis (JE) virus monoclonal (MoAbs) and polyclonal (immune PF) antibodies was evaluated for the detection of viral antigens expressed on the surface of porcine stable kidney cells infected with JE virus (733913, India). The infected cells showed granular immunofluorescence on the surface with both the MoAbs, Hs-1 and Hx-3 and immune PF 24 hr and 48 hr post virus infection. Interestingly, two strains of JE virus viz. Yoken (Japan) and 755468 (India) which did not react with the MoAb Hs-1 in the standard indirect fluorescent antibody (FA) technique, were positive for surface immunofluorescence (IF) with the same MoAb. Thus, the modified technique will be useful for the detection of more labile and conformational-dependent epitopes which might get lost or denatured by prior fixation of infected cells with acetone.
采用抗日本脑炎（JE）病毒单克隆抗体（MoAbs）和多克隆抗体（免疫PF）的改良免疫荧光法，用于检测感染JE病毒（733913，印度）的猪稳定肾细胞表面表达的病毒抗原。病毒感染后24小时和48小时，感染细胞表面用单克隆抗体Hs-1、Hx-3和免疫PF均呈现颗粒状免疫荧光。有趣的是，两株JE病毒，即约肯株（日本）和755468株（印度），在标准间接荧光抗体（FA）技术中不与单克隆抗体Hs-1反应，但用同一单克隆抗体进行表面免疫荧光（IF）检测呈阳性。因此，改良技术将有助于检测更多可能因丙酮预先固定感染细胞而丢失或变性的不稳定和构象依赖性表位。
