Nakajima H, Iwamoto I, Tomoe S, Matsumura R, Tomioka H, Takatsu K, Yoshida S
Second Department of Internal Medicine, Chiba University School of Medicine, Japan.
Am Rev Respir Dis. 1992 Aug;146(2):374-7. doi: 10.1164/ajrccm/146.2.374.
In order to determine the role of CD4+ and CD8+ T-cells and of interleukin-5 (IL-5) in causing antigen-induced eosinophil infiltration into the site of airway late-phase reaction, we examined the effect of the in vivo depletion of CD4+ and CD8+ T-cells on the eosinophil infiltration of the trachea induced by antigen inhalation in mice. We also studied the effect of anti-murine IL-5 monoclonal antibody (mAb) on the antigen-induced eosinophil infiltration in the trachea. The eosinophil infiltration into the trachea of ovalbumin (OVA)-sensitized BALB/c mice began to increase 9 h after OVA inhalation and persisted for more than 48 h. The in vivo depletion of CD4+ T-cells by pretreatment with anti-L3T4 mAb significantly decreased the eosinophil infiltration induced by OVA inhalation in the trachea of sensitized mice. However, the in vivo depletion of CD8+ T-cells by pretreatment with anti-Lyt-2 mAb had no significant effect on OVA-induced eosinophil infiltration in the trachea. Pretreatment with anti-murine IL-5 mAb also decreased OVA-induced eosinophil infiltration in the trachea. In contrast, neither disodium cromoglycate nor a selective antagonist for platelet-activating factor CV-6209 decreased OVA-induced airway eosinophilia in the mouse. Our results provide direct evidence that CD4+ but not CD8+ T-cells mediate antigen-induced eosinophil recruitment in the airways and that IL-5 mediates this eosinophil recruitment.
为了确定CD4+和CD8+ T细胞以及白细胞介素-5（IL-5）在抗原诱导的嗜酸性粒细胞浸润至气道迟发相反应部位中所起的作用，我们研究了体内耗竭CD4+和CD8+ T细胞对小鼠吸入抗原诱导的气管嗜酸性粒细胞浸润的影响。我们还研究了抗小鼠IL-5单克隆抗体（mAb）对气管中抗原诱导的嗜酸性粒细胞浸润的作用。卵清蛋白（OVA）致敏的BALB/c小鼠在吸入OVA后9小时，气管中的嗜酸性粒细胞浸润开始增加，并持续超过48小时。用抗L3T4 mAb预处理在体内耗竭CD4+ T细胞，可显著减少致敏小鼠气管中OVA吸入诱导的嗜酸性粒细胞浸润。然而，用抗Lyt-2 mAb预处理在体内耗竭CD8+ T细胞，对OVA诱导的气管嗜酸性粒细胞浸润没有显著影响。用抗小鼠IL-5 mAb预处理也可减少气管中OVA诱导的嗜酸性粒细胞浸润。相比之下，色甘酸二钠和血小板活化因子CV-6209的选择性拮抗剂均未减少小鼠中OVA诱导的气道嗜酸性粒细胞增多。我们的结果提供了直接证据，即CD4+而非CD8+ T细胞介导气道中抗原诱导的嗜酸性粒细胞募集，且IL-5介导这种嗜酸性粒细胞募集。
