Nakajima H, Nakao A, Watanabe Y, Yoshida S, Iwamoto I
Department of Internal Medicine, Chiba University School of Medicine, Japan.
J Immunol. 1994 Aug 1;153(3):1264-70.
In a murine model of airway late phase reaction, we have previously shown that Ag-induced eosinophil recruitment into the tissue of sensitized mice is mediated by CD4+ T cells and IL-5. To determine whether IFN-alpha regulates Ag-induced eosinophil recruitment into tissue, we studied the effects of rIFN-alpha, endogenous IFN-alpha production induced by poly I.C, and anti-IFN-alpha mAb on the eosinophil infiltration of the trachea induced by Ag inhalation in mice. The i.p. administration of rIFN-alpha prevented Ag-induced eosinophil infiltration in the trachea of sensitized mice. In addition, the inhibitory effect of rIFN-alpha on Ag-induced eosinophil infiltration was not recovered by pretreatment with anti-IFN-gamma mAb. The administration of rIFN-alpha also inhibited Ag-induced CD4+ T cell infiltration in the trachea. However, the administration of rIFN-alpha did not significantly affect blood eosinophil counts nor the number of blood and splenic CD4+ T cells. On the other hand, pretreatment with anti-murine IFN-alpha mAb enhanced Ag-induced eosinophil and CD4+ T cell infiltration in the trachea. Finally, endogenous IFN-alpha/beta production induced by poly I.C also inhibited Ag-induced eosinophil infiltration in the trachea and this inhibition of the eosinophil infiltration was abrogated by pretreatment with anti-IFN-alpha/beta mAb. These results indicate that IFN-alpha suppresses Ag-induced eosinophil and CD4+ T cell recruitment into tissue.
在气道迟发相反应的小鼠模型中，我们先前已表明，抗原诱导的嗜酸性粒细胞募集到致敏小鼠组织中是由CD4 + T细胞和白细胞介素-5介导的。为了确定干扰素-α是否调节抗原诱导的嗜酸性粒细胞募集到组织中，我们研究了重组干扰素-α、聚肌胞苷酸诱导的内源性干扰素-α产生以及抗干扰素-α单克隆抗体对小鼠吸入抗原诱导的气管嗜酸性粒细胞浸润的影响。腹腔注射重组干扰素-α可预防抗原诱导的致敏小鼠气管嗜酸性粒细胞浸润。此外，抗干扰素-γ单克隆抗体预处理不能恢复重组干扰素-α对抗原诱导的嗜酸性粒细胞浸润的抑制作用。重组干扰素-α的给药也抑制了抗原诱导的气管CD4 + T细胞浸润。然而，重组干扰素-α的给药对血液嗜酸性粒细胞计数以及血液和脾脏CD4 + T细胞数量没有显著影响。另一方面，抗小鼠干扰素-α单克隆抗体预处理增强了抗原诱导的气管嗜酸性粒细胞和CD4 + T细胞浸润。最后，聚肌胞苷酸诱导的内源性干扰素-α/β产生也抑制了抗原诱导的气管嗜酸性粒细胞浸润，并且抗干扰素-α/β单克隆抗体预处理可消除这种对嗜酸性粒细胞浸润的抑制作用。这些结果表明，干扰素-α抑制抗原诱导的嗜酸性粒细胞和CD4 + T细胞募集到组织中。
