Harada H, Kimura A, Dong R P, Xu X P, Bhatia K, Sasazuki T
Department of Genetic, Kyushu University, Fukuoka, Japan.
Hum Immunol. 1992 Nov;35(3):173-8. doi: 10.1016/0198-8859(92)90102-s.
We determined the base sequences of the HLA-DPA1 gene from four B-lymphoblastoid cell lines (CB6B, LKT3, AMAI, and T7526) that showed distinct electrophoretic patterns of single-stranded polymerase chain reaction products of the HLA-DPA1 gene. The novel HLA-DPA1 alleles of CB6B, LKT3, AMAI, and T7526 were designated DPA102021, DPA102022, DPA10301, and DPA10401, respectively. Although there was only one base substitution between DPA102021 and DPA102022, the single-strand conformation polymorphism of these two alleles was clearly demonstrated by electrophoresis in a nondenaturing polyacrylamide gel containing 10% glycerol. In addition, we genotyped for the HLA-DPA1 gene of healthy unrelated Oriental individuals--i.e., 227 Japanese, 88 Papua New Guineans, and 41 Buyi-Chinese--to demonstrate the ethnic distribution of the HLA-DPA1 alleles.
我们测定了来自四个B淋巴母细胞系（CB6B、LKT3、AMAI和T7526）的HLA - DPA1基因的碱基序列，这些细胞系的HLA - DPA1基因单链聚合酶链反应产物呈现出不同的电泳图谱。CB6B、LKT3、AMAI和T7526的新型HLA - DPA1等位基因分别命名为DPA102021、DPA102022、DPA10301和DPA10401。尽管DPA102021和DPA102022之间只有一个碱基替换，但在含有10%甘油的非变性聚丙烯酰胺凝胶中进行电泳时，这两个等位基因的单链构象多态性得到了清晰的展示。此外，我们对健康的无关东方个体（即227名日本人、88名巴布亚新几内亚人和41名布依族中国人）的HLA - DPA1基因进行了基因分型，以展示HLA - DPA1等位基因的种族分布。
