D'Argenio G, Ciacci C, Sorrentini I, Ventriglia R, Spagnuolo S, Mattera D, Mellone M C, Iovino P, Mazzacca G
Gastroenterology Unit, II School of Medicine and Surgery, University of Naples, Italy.
Clin Physiol Biochem. 1992;9(2):74-7.
The low serum transglutaminase found in various intestinal disorders (celiac disease and IBD) suggested to us to study the serum and mucosal transglutaminase behaviour in an experimental model of small intestine resection in rats to reduce cellular mass and induce enterocyte hyperproliferation in the proximal part left in continuity. Transglutaminase activity in the intestinal mucosa was significantly higher in resected rats than in control and sham operated animals from days 4 (121 +/- 10 v basal 94 +/- 3 mU/g protein, p < 0.01) to 10 (165 +/- 37 mU/g protein, p < 0.05) after surgery; no significant difference was observed at days 12 and 15 (110 +/- 15 and 105 +/- 23 respectively). Both serum alkaline phosphatase activity (partly produced in enterocytes) and serum transglutaminase were significantly lower in resected rats at each time-point beginning at day 6 (208 +/- 34 v 557 +/- 125 UI and 1.55 +/- 0.11 v 3.78 +/- 0.70 mU/ml, p < 0.001 respectively). These data suggest an involvement of transglutaminase in enterocyte proliferation and confirm the association between reduced intestinal mass and low levels of the enzyme in serum.
在各种肠道疾病（乳糜泻和炎症性肠病）中发现的低血清转谷氨酰胺酶，促使我们在大鼠小肠切除实验模型中研究血清和黏膜转谷氨酰胺酶的行为，以减少细胞数量并诱导留存的近端肠段肠上皮细胞过度增殖。术后第4天（切除组为121±10，对照组和假手术组基础值为94±3 mU/g蛋白，p<0.01）至第10天（165±37 mU/g蛋白，p<0.05），切除组大鼠肠黏膜中的转谷氨酰胺酶活性显著高于对照组和假手术组动物；术后第12天和第15天未观察到显著差异（分别为110±15和105±23）。从第6天开始，切除组大鼠在每个时间点的血清碱性磷酸酶活性（部分由肠上皮细胞产生）和血清转谷氨酰胺酶均显著低于对照组（分别为208±34对557±125 UI，1.55±0.11对3.78±0.70 mU/ml，p均<0.001）。这些数据表明转谷氨酰胺酶参与了肠上皮细胞增殖，并证实了肠质量减少与血清中该酶水平降低之间的关联。
