三种血清学检测方法用于检测Q热患者抗抗体的比较。
Comparison of Three Serologic Tests for the Detection of Anti- Antibodies in Patients with Q Fever.
作者信息
França Danilo Alves de, Mioni Mateus de Souza Ribeiro, Fornazari Felipe, Rodrigues Nássarah Jabur Lot, Polido Lucas Roberto Ferreira, Appolinario Camila Michele, Ribeiro Bruna Letícia Devidé, Duré Ana Íris de Lima, Silva Marcos Vinicius Ferreira, Richini-Pereira Virgínia Bodelão, Langoni Helio, Megid Jane
机构信息
Department of Veterinary Hygiene and Public Health, São Paulo State University, Botucatu 05508-220, Brazil.
Botucatu Medical School, São Paulo State University, Botucatu 05508-220, Brazil.
出版信息
Pathogens. 2023 Jun 26;12(7):873. doi: 10.3390/pathogens12070873.
The performance of a commercial immunofluorescence assay (IFA commercial), an immunofluorescence assay (IFA ) and an indirect enzyme-linked immunosorbent assay (ELISA) were evaluated in the detection of antibodies anti- in the serum of Q fever patients and persons without the disease. For the study, seropositive and seronegative samples for Q fever ( = 200) from a serum bank of the Instituto Adolfo Lutz in Brazil were used. Commercial IFA was considered in this study as the gold standard for diagnosing Q fever. The IFA demonstrated good agreement with the commercial test, showing high sensitivity (91%) and specificity (97%) compared to the gold standard, with a Kappa coefficient of 0.8954. The indirect ELISA test showed lower agreement with the gold standard, showing low sensitivity (67%), although the specificity of the technique was high (97%) and the Kappa coefficient was moderate (0.6631). IFA is an excellent alternative for diagnosing Q fever.
评估了一种商业免疫荧光测定法（商业免疫荧光测定法）、一种免疫荧光测定法（免疫荧光测定法）和一种间接酶联免疫吸附测定法（ELISA）在检测Q热患者和非Q热患者血清中抗Q热抗体方面的性能。在该研究中，使用了巴西阿道夫·卢茨研究所血清库中200份Q热血清学阳性和阴性样本。本研究将商业免疫荧光测定法视为诊断Q热的金标准。免疫荧光测定法与商业检测显示出良好的一致性，与金标准相比，灵敏度高（91%），特异性高（97%），kappa系数为0.8954。间接ELISA检测与金标准的一致性较低，灵敏度低（67%），尽管该技术的特异性高（97%），kappa系数中等（0.6631）。免疫荧光测定法是诊断Q热的一种优秀替代方法。
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