Study of the priming effect of interferon in L cells. II. Metabolic requirements for the development of primed state.

Actinomycin D at a concentration inhibitory for nucleolar RNA synthesis did not influence the development of priming. Higher concentrations of the drug and long-term alpha-amanitin or cycloheximide pretreatments, inhibitory for heterogenous nuclear RNA synthesis, prevented the establishment of the primed state. The inhibition of mitochondrial DNA and protein synthesis did not inhibit the priming effect of interferon. Attempts to prime cells with pretreatments other than interferon were not successful.