HORTON E W
Br J Pharmacol Chemother. 1959 Mar;14(1):125-32. doi: 10.1111/j.1476-5381.1959.tb00938.x.
It has been shown that the isolated rat duodenum relaxes in the presence of low concentrations of plasma and urinary kinin. The tissue is at least as sensitive as the rat uterus. Vasopressin and oxytocin, in large doses, also caused relaxation of the duodenum whereas acetylcholine, substance P and 5-hydroxytryptamine caused contraction. It was concluded that if an extract is assayed on the rat uterus and the rat duodenum in parallel using plasma kinin as a standard, and the results agree, this is good evidence that the active principle being estimated is a kinin. This method is therefore both sensitive and specific for kinin estimations, but it will not distinguish between kinins of different origin. The urinary excretion of kinin in 14 healthy adults was found to be fairly constant. The minute output was unaffected by the rate of urine formation, urinary pH, or time of day. There was no increase during sweating or salivation.
已表明，在低浓度血浆和尿激肽存在的情况下，离体大鼠十二指肠会松弛。该组织至少与大鼠子宫一样敏感。大剂量的血管加压素和催产素也会导致十二指肠松弛，而乙酰胆碱、P物质和5-羟色胺则会导致收缩。得出的结论是，如果以血浆激肽为标准，同时在大鼠子宫和大鼠十二指肠上对提取物进行检测，且结果一致，那么这就是所测定的活性成分是激肽的有力证据。因此，该方法对激肽的测定既敏感又特异，但无法区分不同来源的激肽。发现14名健康成年人的尿激肽排泄量相当恒定。每分钟的排泄量不受尿液生成速率、尿液pH值或一天中时间的影响。出汗或流涎时也没有增加。
