Quinton P M, Tormey J M
J Membr Biol. 1976 Nov 29;29(4):383-99. doi: 10.1007/BF01868972.
Na/K-ATPase sites in both the secretory and reabsorptive epithelia of isolated and microperfused human eccrine sweat glands are localized cytologically. Localization was accomplished through autoradiography of bound 3H-ouabain, a specific inhibitor of the enzyme. Ouabain binding characteristics were determined to ensure maximum specific binding. Enzyme sites are localized only on the basolateral surface of both epithelia in spite of the fact that sodium transport is reversed, i.e., secretory: blood to lumen and reabsorptive: lumen to blood. In view of these findings and in comparison with other recent observations, the role of Na/K-ATPase in secretory electrolyte transport is questioned.
通过细胞定位法确定了分离并经微灌注的人外泌汗腺分泌和重吸收上皮细胞中的钠钾ATP酶位点。通过对该酶的特异性抑制剂——结合型3H-哇巴因进行放射自显影来实现定位。测定了哇巴因的结合特性以确保最大程度的特异性结合。尽管钠的转运方向相反，即分泌时：从血液到管腔，重吸收时：从管腔到血液，但酶位点仅定位在两种上皮细胞的基底外侧表面。鉴于这些发现并与其他近期观察结果相比较，钠钾ATP酶在分泌性电解质转运中的作用受到质疑。
