Anversa P, Loud A V, Vitali-Mazza L
Lab Invest. 1976 Nov;35(5):475-83.
The ultrastructural composition of left ventricular myocardium and myocytes has been investigated at 20 hours after the surgical production of a subdiaphragmatic aortic stenosis in the adult rat. Morphometric analysis shows a 20% increase in the size of myocytes resulting principally from a 36% increase in mitochondrial volume and a 78% increase in the volumes occupied by smooth endoplasmic reticulum and matrix. Myofibrillar volume is increased only 4%. Measurements of the size and shape of individual mitochondria indicate that the augmentation of this compartment is wholly accountable by the enlargement of preexisting mitochondria. Quantitative autoradiographic analysis following a 2-hour pulse labeling with 3H-leucine shows that the rate of incorporation of newly synthesized proteins is practically uniform throughout all structural components of the sarcoplasm 18 to 20 hours after aortic banding. The conclusion is reached that this interval of hypertrophic response is a transition period characterized by an increasing rate of synthesis of contractile proteins overtaking a previously greater rate of mitochondrial synthesis.
在成年大鼠膈下主动脉狭窄手术制造后20小时，对左心室心肌和心肌细胞的超微结构组成进行了研究。形态计量分析显示，心肌细胞大小增加了20%，这主要是由于线粒体体积增加了36%，以及滑面内质网和基质所占体积增加了78%。肌原纤维体积仅增加了4%。对单个线粒体大小和形状的测量表明，该隔室的增大完全是由现有线粒体的增大所致。在用3H-亮氨酸进行2小时脉冲标记后的定量放射自显影分析表明，主动脉结扎后18至20小时，新合成蛋白质的掺入率在肌浆的所有结构成分中实际上是均匀的。得出的结论是，这种肥厚反应的间隔是一个过渡期，其特征是收缩蛋白合成速率增加，超过了先前更高的线粒体合成速率。
