Kordek R, Biernat W, Kupnicka D, Woźniak L
Department of Tumor Pathology, Medical University, Lódź.
Pol J Pathol. 1994;45(4):279-81.
The presence of Epstein-Barr virus (EBV) correlates with some cases of Hodgkin's disease (HD), and its latent membrane protein (LMP) has oncogenic potential by inducing expression of bcl-2 protein. Bcl-2 confers a longer half-life to the cell, which overexpresses it. As the translocation t(14,18), which is most often found in follicular lymphomas, and leads to overexpression of bcl-2, has also been reported in HD, it is possible that there is a correlation between these events in this entity. We stained immunohistochemically 40 cases of HD for the presence of bcl-2 and EBV-LMP. Bcl-2 positivity within reactive lymphocytes was revealed in 29 cases. In five of these cases a week, positive reaction in cytoplasm of Reed-Sternberg cells was observed (one mixed cellularity and four nodular sclerosis cases). The EBV-LMP immunopositivity was observed in 16 of these 29 cases (ten MC and six NS cases). The simultaneous presence of bcl-2 protein and EBV-LMP was found in two cases, the remaining three bcl-2-positive cases did not have EBV-LMP. These results do not support the hypothesis of the correlation between the expression of bcl-2 protein and the presence of EBV-LMP in the pathogenesis of HD as the LMP-dependent stimulation of bcl-2 oncogene.
爱泼斯坦-巴尔病毒（EBV）的存在与某些霍奇金淋巴瘤（HD）病例相关，其潜伏膜蛋白（LMP）通过诱导bcl-2蛋白表达而具有致癌潜力。Bcl-2赋予过表达它的细胞更长的半衰期。由于在HD中也报道了最常见于滤泡性淋巴瘤且导致bcl-2过表达的t(14,18)易位，因此在该实体中这些事件之间可能存在相关性。我们对40例HD病例进行免疫组织化学染色，检测bcl-2和EBV-LMP的存在情况。29例病例的反应性淋巴细胞内显示bcl-2阳性。在其中5例病例中，观察到里德-斯腾伯格细胞胞质呈弱阳性反应（1例混合细胞型和4例结节硬化型病例）。在这29例病例中的16例（10例混合细胞型和6例结节硬化型病例）观察到EBV-LMP免疫阳性。在2例病例中发现bcl-2蛋白和EBV-LMP同时存在，其余3例bcl-2阳性病例未检测到EBV-LMP。这些结果不支持在HD发病机制中bcl-2蛋白表达与EBV-LMP存在之间存在相关性的假设，即LMP依赖的bcl-2癌基因刺激。
