BOHME D H, SCHNEIDER H A, LEE J M
J Exp Med. 1959 Jul 1;110(1):9-26. doi: 10.1084/jem.110.1.9.
Susceptible (BSVS) and resistant (BRVR) mice were experimentally infected with Salmonella typhimurium. The double strain inoculation technique was used in which both avirulent and virulent representatives of S. typhimurium were admitted into the hosts. The BSVS mice succumbed without exception while the BRVR mice survived to 52 per cent. No deaths occurred during the experimental period in non-infected control mice. Parallel to the survivorship test, and concurrent with it, the activity of the reticulo-endothelial system (RES) was measured at frequent intervals in identically infected BSVS and BRVR mice and in their non-infected controls. For this measurement the carbon clearance method was used. No pre-infection difference of activity of the RES could be discerned in susceptible BSVS mice vs. resistant BRVR. Following infection, increases in the RES activity were detected in about the same magnitude in both mouse stocks. However, a difference was found in the time of onset. The susceptible animals showed an early and short increase in the activity of the RES, followed by a drop to control levels at the time of death. The resistant group exhibited a considerably delayed, but significant increase in RES activity, which returned to control levels approximately 4 weeks after infection. The absolute white blood count did not undergo significant change in either of the two infected groups, but the susceptible animals showed a relative increase of their neutrophils at the expense of their lymphocytes. Extensive anatomical changes were observed in both mouse strains, mainly confined to liver and spleen. These consisted of stasis, swelling of Kupffer cells, necrotic foci, histiocytic-monocytic nodules, widespread thrombosis of branches of the portal and splenic veins, and extensive areas of necrosis. These changes appeared earlier in susceptible than in resistant animals. The implications of these findings are discussed.
将易感性（BSVS）小鼠和抗性（BRVR）小鼠用鼠伤寒沙门氏菌进行实验性感染。采用双菌株接种技术，将无毒力和有毒力的鼠伤寒沙门氏菌代表菌株都接种到宿主中。BSVS小鼠无一例外均死亡，而BRVR小鼠存活率达52%。在实验期间，未感染的对照小鼠未出现死亡。与存活试验并行且同时进行的是，在相同感染的BSVS和BRVR小鼠及其未感染的对照小鼠中，定期测量网状内皮系统（RES）的活性。为此采用了碳清除法。在易感性BSVS小鼠与抗性BRVR小鼠之间，未发现感染前RES活性存在差异。感染后，在两种小鼠品系中均检测到RES活性有大致相同程度的增加。然而，在起始时间上发现了差异。易感动物RES活性早期短暂升高，随后在死亡时降至对照水平。抗性组RES活性升高明显延迟，但幅度显著，在感染后约4周恢复到对照水平。两个感染组的绝对白细胞计数均未发生显著变化，但易感动物的中性粒细胞相对增加，淋巴细胞相对减少。在两种小鼠品系中均观察到广泛的解剖学变化，主要局限于肝脏和脾脏。这些变化包括淤血、库普弗细胞肿胀、坏死灶、组织细胞 - 单核细胞结节、门静脉和脾静脉分支广泛血栓形成以及大片坏死区域。这些变化在易感动物中比在抗性动物中出现得更早。讨论了这些发现的意义。
