Miner J N, Hruby D E
Department of Microbiology, Oregon State University, Corvallis 97331.
Trends Biotechnol. 1990 Jan;8(1):20-5. doi: 10.1016/0167-7799(90)90126-i.
Continued advances in genetic engineering have made possible the high-level expression of correctly processed cellular, viral and bacterial polypeptides. This article focuses on viral expression vectors and, more specifically, the vaccinia virus expression system. Vaccinia virus has been used to express a variety of proteins with useful immunogenic, catalytic or pharmaceutical properties. We discuss briefly the biology of vaccinia and its significance in the use of vaccinia as an expression vector, the variety of vaccinia systems currently in use and, finally, we summarize some recent developments which bode well for future applications of vaccinia virus technology.
基因工程的持续进步使得正确加工的细胞、病毒和细菌多肽得以高水平表达。本文重点关注病毒表达载体，更具体地说是痘苗病毒表达系统。痘苗病毒已被用于表达多种具有有用免疫原性、催化或药物特性的蛋白质。我们简要讨论痘苗的生物学特性及其作为表达载体使用的意义、目前使用的各种痘苗系统，最后总结一些近期的进展，这些进展对痘苗病毒技术的未来应用预示着良好的前景。
