Hruby D E, Thomas G
Department of Microbiology, Oregon State University, Corvallis 97331.
Pharm Res. 1987 Apr;4(2):92-7. doi: 10.1023/a:1016454600151.
Recent technological advancements have fostered the continued development of vaccinia virus as an efficient eukaryotic cloning and expression vector system. Genetically engineered vaccinia virus strains have been constructed for use (i) as recombinant vaccines for the prophylaxis of infectious disease, (ii) in producing significant quantities of biologically active polypeptide factors or enzymes, and (iii) as basic research tools with which to investigate primary structure-function relationships between proteins and their catalytic activities. This review examines the basic vaccinia vector system, its advantages and limitations, and current areas of research. As a specific example of the power and utility of this approach, attention is focused on the application of this technology to the field of neurobiology, specifically the use of recombinant vaccinia to study the expression, processing, and transport of cellular neuropeptides.
最近的技术进步推动了痘苗病毒作为一种高效的真核克隆和表达载体系统的持续发展。已经构建了基因工程痘苗病毒株，用于：（i）作为预防传染病的重组疫苗；（ii）大量生产生物活性多肽因子或酶；以及（iii）作为研究蛋白质与其催化活性之间一级结构 - 功能关系的基础研究工具。本文综述了基本的痘苗载体系统、其优点和局限性以及当前的研究领域。作为这种方法的强大功能和实用性的一个具体例子，重点关注了该技术在神经生物学领域的应用，特别是利用重组痘苗研究细胞神经肽的表达、加工和运输。
