一种从野生型革兰氏阴性细菌中快速分离质粒DNA用于质粒限制性图谱分析的改进方法。
An improved method for rapid isolation of plasmid DNA from wild-type gram-negative bacteria for plasmid restriction profile analysis.
作者信息
Olsen J E
机构信息
Department of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Frederiksberg C., Denmark.
出版信息
Lett Appl Microbiol. 1990 May;10(5):209-12. doi: 10.1111/j.1472-765x.1990.tb01335.x.
Abstract
An improved method for the isolation of large and small plasmids from wild-type Gram-negative bacteria has been developed. The protocol combines the lysis and purification procedures of two popular plasmid isolation methods, and produces DNA sufficiently pure for restriction enzyme digestion in less than three hours.
摘要
已开发出一种从野生型革兰氏阴性细菌中分离大小质粒的改进方法。该方案结合了两种常用质粒分离方法的裂解和纯化程序，能在不到三小时内产生纯度足以用于限制性内切酶消化的DNA。
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