Bonhoff A, Gellersen B, Held K R, Bohnet H G
Institute of Hormone and Fertility Research, Hamburg, FRG.
Cytotechnology. 1990 Jan;3(1):43-50. doi: 10.1007/BF00365265.
The IM-9-P cell line is a variant of the human B-lymphoblastoid cell line IM-9 which ectopically secretes prolactin (hPRL). The heterogeneous line IM-9-P and three sublines of clonal origin, two of them positive and one negative for PRL gene expression, were subjected to cytogenetic analysis and compared with the reference line IM-9 which showed a normal female diploid karyotype. G-banding revealed several rearrangements in the chromosomes. Nine altered chromosomes including one stable marker chromosome were common to all analysed karyotypes of IM-9-P cells and their clones. A second marker chromosome 'mar2' occurred only in the karyotypes of the hPRL producing clones, but not in the non-producing clone. None of the visible alterations involve chromosome 6 which carries the PRL gene in humans.
IM-9-P细胞系是人类B淋巴母细胞系IM-9的一个变体，它异位分泌催乳素（hPRL）。对异质细胞系IM-9-P及其三个克隆起源的亚系进行了细胞遗传学分析，其中两个亚系PRL基因表达呈阳性，一个呈阴性，并与显示正常女性二倍体核型的参照细胞系IM-9进行了比较。G显带揭示了染色体中的几种重排。九条改变的染色体，包括一条稳定的标记染色体，在IM-9-P细胞及其克隆的所有分析核型中都很常见。第二条标记染色体“mar2”仅出现在产生hPRL的克隆的核型中，而不出现在不产生hPRL的克隆中。所有可见的改变均不涉及人类携带PRL基因的6号染色体。
