一种用于稳定表达乙型肝炎表面抗原的酵母系统。

A yeast system for stable expression of hepatitis B surface antigen.

作者信息

Shiosaki K, Miyatsu Y, Takahara K, Mizokami H, Hamada F, Matsubara K

机构信息

Institute for Molecular and Cellular Biology, Osaka University, Japan.

出版信息

J Biotechnol. 1990 Jun;14(3-4):411-22. doi: 10.1016/0168-1656(90)90122-r.

DOI:10.1016/0168-1656(90)90122-r

PMID:1366913

Abstract

We have constructed a yeast strain that has integrated into its chromosomal ribosomal RNA gene site two copies of Hepatitis B virus surface (HBS) antigen gene under the control of the yeast (Saccharomyces cerevisiae) glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter and terminator. The level of expression of HBS gene was low in the strain, but upon chemical and physical mutageneses, in combination with an immunological screening procedure, a mutant clone which expressed HBS protein at a high level was obtained. This mutant strain produces HBS antigen stably under non-selective conditions.

摘要

我们构建了一种酵母菌株，该菌株已将两个乙型肝炎病毒表面（HBS）抗原基因拷贝整合到其染色体核糖体RNA基因位点，这两个基因拷贝受酵母（酿酒酵母）甘油醛-3-磷酸脱氢酶（GAP）启动子和终止子的控制。该菌株中HBS基因的表达水平较低，但经过化学和物理诱变，并结合免疫筛选程序，获得了一个高水平表达HBS蛋白的突变克隆。这种突变菌株在非选择性条件下能稳定产生HBS抗原。