Mancuso A, Fernandez E J, Blanch H W, Clark D S
Department of Chemical Engineering, University of California, Berkeley 94720.
Biotechnology (N Y). 1990 Dec;8(12):1282-5. doi: 10.1038/nbt1290-1282.
We have developed a technique for determining cell concentration in a hollow fiber bioreactor based on 23Na nuclear magnetic resonance (NMR) spectroscopy. Cell concentrations determined with this method agreed closely with concentrations calculated from 31P NMR nucleoside triphosphate (NTP) measurements and oxygen consumption rate measurements. Oxygen transfer limitations, which can complicate cell mass determinations based on oxygen consumption rates, were shown to be negligible for the bioreactor used. Specific antibody production rates in hollow fiber culture, calculated from these cell number estimates, were similar to those found in suspension culture for this cell line.
我们开发了一种基于23Na核磁共振（NMR）光谱测定中空纤维生物反应器中细胞浓度的技术。用该方法测定的细胞浓度与通过31P NMR核苷三磷酸（NTP）测量和耗氧率测量计算出的浓度非常吻合。对于所使用的生物反应器，耗氧限制（这可能会使基于耗氧率的细胞质量测定变得复杂）被证明是可以忽略不计的。根据这些细胞数量估计值计算得出的中空纤维培养物中的特异性抗体产生率，与该细胞系在悬浮培养中的情况相似。
