Su Wei Wen
Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Hawaii 96822, USA.
Microb Cell Fact. 2005 Apr 25;4(1):12. doi: 10.1186/1475-2859-4-12.
Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GFP) from Aequorea victoria, a variety of other fluorescent proteins have been discovered that display a wide range of spectral properties. Synthetic variants have also been developed to overcome limitations associated with their wild-type counterparts. Having a large repertoire of fluorescent proteins with diverse traits opens new opportunities for rapid monitoring and optimization of recombinant protein production.
荧光蛋白是通过基因编码的、用途广泛的报告分子，可用于监测重组蛋白生产的各个方面。除了来自维多利亚水母的广受欢迎的绿色荧光蛋白（GFP）外，还发现了多种其他荧光蛋白，它们具有广泛的光谱特性。还开发了合成变体以克服与其野生型对应物相关的局限性。拥有大量具有不同特性的荧光蛋白为重组蛋白生产的快速监测和优化提供了新机会。
