里氏木霉纤维素酶在含葡萄糖培养基上的生产。
Production of Trichoderma reesei cellulases on glucose-containing media.
作者信息
Nakari-Setälä T, Penttilä M
机构信息
VTT Biotechnology and Food Research, Espoo, Finland.
出版信息
Appl Environ Microbiol. 1995 Oct;61(10):3650-5. doi: 10.1128/aem.61.10.3650-3655.1995.
Abstract
The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The highest amounts of cellobiohydrolase I and the endoglucanase I core, being 50 to 100 mg/liter, accounted for more than half of the total protein secreted by the fungus. The levels obtained with the tef1 promoter were 20 to 50 times lower.
摘要
丝状真菌里氏木霉在含葡萄糖的培养基上生长时，会将活性纤维二糖水解酶I和内切葡聚糖酶I催化核心结构域分泌到培养基中。这些蛋白质的表达由延伸因子1α（tef1）的启动子和未鉴定的cDNA1基因的启动子驱动。cDNA1启动子的产量最高。纤维二糖水解酶I和内切葡聚糖酶I核心的最高产量为50至100毫克/升，占真菌分泌的总蛋白的一半以上。tef1启动子获得的产量低20至50倍。
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