Russu I M
Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06457.
Trends Biotechnol. 1991 Mar;9(3):96-104. doi: 10.1016/0167-7799(91)90032-d.
NMR spectroscopy is emerging as a powerful tool in molecular biology and biotechnology; one aspect of which is the use of one- and two-dimensional NMR methodologies to investigate the interactions of proteins with DNA. The dynamic and structural information which NMR can provide, on the changes in conformation and molecular flexibility, complements X-ray crystallography data and enables mechanistic models of DNA-protein interactions to be formulated.
核磁共振光谱正成为分子生物学和生物技术领域一种强大的工具；其中一个方面是利用一维和二维核磁共振方法来研究蛋白质与DNA的相互作用。核磁共振能够提供的关于构象变化和分子柔韧性的动态和结构信息，补充了X射线晶体学数据，并有助于构建DNA-蛋白质相互作用的机制模型。
