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Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining.

作者信息

Nikolai T J, Peshwa M V, Goetghebeur S, Hu W S

机构信息

Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455.

出版信息

Cytotechnology. 1991 Feb;5(2):141-6. doi: 10.1007/BF00365430.

DOI:10.1007/BF00365430
PMID:1367155
Abstract

Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphology and viability of cells on various microcarriers.

摘要

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本文引用的文献

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Attachment and growth of mammalian cells on microcarriers with different ion exchange capacities.哺乳动物细胞在具有不同离子交换能力的微载体上的附着与生长
Biotechnol Bioeng. 1987 Jun;29(9):1155-63. doi: 10.1002/bit.260290917.
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Serial propagation of mammalian cells on microcarriers.哺乳动物细胞在微载体上的连续传代培养
Biotechnol Bioeng. 1985 Oct;27(10):1466-76. doi: 10.1002/bit.260271011.
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Growth of three established cell lines on glass microcarriers.三种已建立的细胞系在玻璃微载体上的生长情况。
悬浮分批培养中动物细胞天然聚集体的变化。
Appl Microbiol Biotechnol. 1994 Apr;41(2):203-9. doi: 10.1007/BF00186960.
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Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: an in vitro system with application to the bioartificial liver. Rat hepatocytes cultured in cylindrical collagen gels.包裹在圆柱形胶原凝胶中的大鼠肝细胞原代培养：一种应用于生物人工肝的体外系统。在圆柱形胶原凝胶中培养的大鼠肝细胞。
Cytotechnology. 1992;10(3):205-15. doi: 10.1007/BF00146671.
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Cultivation of anchorage-dependent animal cells in microsphere-induced aggregate culture.
Appl Microbiol Biotechnol. 1991 Mar;34(6):735-41. doi: 10.1007/BF00169343.
Biotechnol Bioeng. 1983 May;25(5):1359-72. doi: 10.1002/bit.260250515.
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Factors affecting cell attachment, spreading, and growth on derivatized microcarriers. I. Establishment of working system and effect of the type of the amino-charged groups.影响细胞在衍生化微载体上附着、铺展和生长的因素。I. 工作体系的建立及氨基带电基团类型的影响
Biotechnol Bioeng. 1983 Feb;25(2):469-80. doi: 10.1002/bit.260250213.
5
Growth of anchorage dependent mammalian cells on glycine-derivatized polystyrene in suspension culture.贴壁依赖型哺乳动物细胞在悬浮培养中于甘氨酸衍生化聚苯乙烯上的生长。
In Vitro. 1981 Oct;17(10):901-6. doi: 10.1007/BF02618286.
6
Biosilon a new microcarrier.Biosilon是一种新型微载体。
Dev Biol Stand. 1980;46:125-9.
7
Cell growth on liquid microcarriers.液体微载体上的细胞生长
Science. 1983 Mar 25;219(4591):1448-9. doi: 10.1126/science.6828872.
8
The use of Cytodex 3 microcarriers and reduced-serum media for the production of nerve growth promoters from chicken heart cells.使用Cytodex 3微载体和低血清培养基从鸡心脏细胞生产神经生长促进剂。
Dev Biol Stand. 1983;55:43-51.
9
A rapid, quantitative fluorescence assay for cell damage by cytotoxic antibodies.一种用于检测细胞毒性抗体所致细胞损伤的快速定量荧光测定法。
J Immunol. 1970 May;104(5):1303-6.
10
An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide.一种通过荧光素二乙酸酯-碘化丙啶同时染色来测定细胞活力的改进方法。
J Histochem Cytochem. 1985 Jan;33(1):77-9. doi: 10.1177/33.1.2578146.