监测旨在代表工业生产菌株的重组大肠杆菌在土壤微观环境中的存活情况和基因转移。
Monitoring survival and gene transfer in soil microcosms of recombinant Escherichia coli designed to represent an industrial production strain.
作者信息
Henschke R B, Henschke E J, Schmidt F R
机构信息
Bundesforschungsanstalt für Landwirtschaft (FAL), Institut für Bodenbiologie, Braunschweig, Federal Republic of Germany.
出版信息
Appl Microbiol Biotechnol. 1991 May;35(2):247-52. doi: 10.1007/BF00184696.
A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using different monitoring methods, including the polymerase-chain reaction, to amplify and detect the specific rDNA. Depending on nutritional status, both the GEM and its rDNA had disappeared within 16 (amended soil) or 28 days (non-amended soil) with a limit of detection of 5 cells/g soil and 20 fg DNA/g soil.
设计了一种基因工程微生物（GEM）来代表工业上用于生产生物材料的细菌菌株。重组DNA位于安全质粒（pUC19）上。通过使用不同的监测方法，包括聚合酶链反应，来扩增和检测特定的rDNA，从而在土壤微观世界中确定GEM及其重组DNA（rDNA）的存活和持久性。根据营养状况，GEM及其rDNA在16天（改良土壤）或28天（未改良土壤）内消失，检测限为5个细胞/克土壤和20 fg DNA/克土壤。
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