Ebert K M, Selgrath J P, DiTullio P, Denman J, Smith T E, Memon M A, Schindler J E, Monastersky G M, Vitale J A, Gordon K
Tufts University School of Veterinary Medicine, North Grafton, MA 01536-1895.
Biotechnology (N Y). 1991 Sep;9(9):835-8. doi: 10.1038/nbt0991-835.
We report the first successful production of transgenic goats that express a heterologous protein in their milk. The production of a glycosylation variant of human tPA (LAtPA--longer acting tissue plasminogen activator) from an expression vector containing the murine whey acid promoter (WAP) operatively linked to the cDNA of a modified version of human tPA was examined in transgenic dairy goats. Two transgenic goats were identified from 29 animals born. The first animal, a female, was mated and allowed to carry the pregnancy to term. Milk was obtained upon parturition and was shown to contain enzymatically active LAtPA at a concentration of 3 micrograms/ml.
我们报道了首例成功培育出能在乳汁中表达异源蛋白的转基因山羊。在转基因奶山羊中，研究了从一个含有与修饰版人组织型纤溶酶原激活剂（tPA）的互补DNA（cDNA）有效连接的鼠乳清酸蛋白启动子（WAP）的表达载体中生产人tPA的糖基化变体（LAtPA，即长效组织型纤溶酶原激活剂）。在出生的29只动物中鉴定出了两只转基因山羊。第一只动物是一只雌性，使其交配并妊娠至足月。分娩时获取了乳汁，结果显示乳汁中含有浓度为3微克/毫升的具有酶活性的LAtPA。
