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自发和热诱导的光子发射:检测和量化植物氧化应激的新方法。

Spontaneous and thermoinduced photon emission: new methods to detect and quantify oxidative stress in plants.

作者信息

Havaux Michel

机构信息

CEA/Cadarache, DEVM, DSV, Laboratoire d'Ecophysiologie de la Photosynthèse, UMR CEA-CNRS 163, Univ. Méditérranée-CEA 1000, F-13108 Saint-Paul-lez-Durance, France.

出版信息

Trends Plant Sci. 2003 Sep;8(9):409-13. doi: 10.1016/S1360-1385(03)00185-7.

Abstract

Peroxidation of polyunsaturated fatty acids is one of the main events triggered by oxidative stress in cells. Some lipid peroxidation products are light-emitting species, and their luminescence can be used as an internal marker of oxidative stress. However, this spontaneous chemiluminescence is weak and difficult to measure. Recent studies have shown that an alternative approach that involves measuring thermoluminescence bands at high temperature (in the range 80-150 degrees C) is a simple way of detecting and quantifying lipid peroxidative damage and oxidative stress in plants.

摘要

多不饱和脂肪酸的过氧化是细胞中氧化应激引发的主要事件之一。一些脂质过氧化产物是发光物质,它们的发光可作为氧化应激的一种内部标志物。然而,这种自发化学发光很微弱且难以测量。最近的研究表明,一种涉及测量高温(80 - 150摄氏度范围)热发光带的替代方法,是检测和量化植物中脂质过氧化损伤及氧化应激的简便方式。

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