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自体荧光成像:一种用于绘制氧化应激图谱的非侵入性工具。

Autoluminescence imaging: a non-invasive tool for mapping oxidative stress.

作者信息

Havaux Michel, Triantaphylidès Christian, Genty Bernard

机构信息

Laboratoire d'Ecophysiologie Moléculaire des Plantes, UMR 6191 CEA-CNRS-Aix Marseille University, F-13108 Saint-Paul-lez-Durance, France.

出版信息

Trends Plant Sci. 2006 Oct;11(10):480-4. doi: 10.1016/j.tplants.2006.08.001. Epub 2006 Sep 7.

Abstract

Living organisms spontaneously emit faint light, and this autoluminescence is stimulated in response to many stresses. This phenomenon is attributable to the endogenous production of excited states during oxidative reactions, particularly during peroxidation of lipids, which generates light-emitting molecules such as triplet carbonyls and singlet oxygen. Using highly sensitive cameras, it is now possible to remotely image spontaneous luminescence with a good spatial resolution, providing a new non-invasive tool for mapping oxidative stress and lipid peroxidation in plants.

摘要

活生物体能自发地发出微弱的光,并且这种自体发光会因多种应激而被激发。这种现象归因于氧化反应过程中激发态的内源性产生,特别是在脂质过氧化过程中,脂质过氧化会产生诸如三线态羰基和单线态氧等发光分子。使用高灵敏度相机,现在可以以良好的空间分辨率对自发发光进行远程成像,为绘制植物中的氧化应激和脂质过氧化情况提供了一种新的非侵入性工具。

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