Tzortzis G, Goulas A K, Baillon M-L A, Gibson G R, Rastall R A
School of Food Biosciences, The University of Reading, Whiteknights, PO Box 226 Reading, RG6 6AP, UK.
Appl Microbiol Biotechnol. 2004 Mar;64(1):106-11. doi: 10.1007/s00253-003-1427-z. Epub 2003 Sep 6.
Stirred, pH-controlled anaerobic batch cultures were used to evaluate the in vitro utilisation by canine gut microflora of novel alpha-galactooligosaccharides synthesised with an enzyme extract from a canine Lactobacillus reuteri strain. Fructooligosaccharides (FOS), melibiose and raffinose were used as reference carbohydrates for the prebiotic properties of the synthesised oligosaccharide (galactosyl melibiose mixture-GMM). Addition of Lactobacillus acidophilus was used as control for the evaluation of the synbiotic properties of the oligosaccharide with L. reuteri. Populations of predominant gut bacterial groups were monitored over 48 h of batch culture by fluorescent in situ hybridisation, and short-chain fatty acid (SCFA) production was measured. GMM showed a higher increase in bifidobacteria and lactobacilli population number and size as well as a higher decrease in clostridia population number and size compared to the commercial prebiotics (FOS, melibiose, raffinose). This prebiotic effect was further increased by the addition of L. reuteri followed by a change in the SCFA production pattern compared to GMM alone or GMM with L. acidophilus. The observed change in SCFA production was in accordance with the fermentation properties of L. reuteri, suggesting that the novel synbiotic had a significant effect on the canine gut microflora fermentation.
采用搅拌式、pH值控制的厌氧分批培养法，评估犬肠道微生物群对用犬源罗伊氏乳杆菌菌株的酶提取物合成的新型α-低聚半乳糖的体外利用情况。低聚果糖（FOS）、蜜二糖和棉子糖用作合成低聚糖（半乳糖基蜜二糖混合物-GMM）益生元特性的参考碳水化合物。添加嗜酸乳杆菌作为对照，以评估该低聚糖与罗伊氏乳杆菌的合生元特性。通过荧光原位杂交在分批培养的48小时内监测主要肠道细菌群的数量，并测量短链脂肪酸（SCFA）的产生。与商业益生元（FOS、蜜二糖、棉子糖）相比，GMM显示双歧杆菌和乳酸杆菌的数量和大小增加更多，梭菌的数量和大小减少更多。与单独的GMM或添加嗜酸乳杆菌的GMM相比，添加罗伊氏乳杆菌后，这种益生元效应进一步增强，随后SCFA产生模式发生变化。观察到的SCFA产生变化与罗伊氏乳杆菌的发酵特性一致，表明这种新型合生元对犬肠道微生物群发酵有显著影响。
