Fava F, Di Gioia D, Marchetti L, Quattroni G
Department of Applied Chemistry and Material Science, University of Bologna, Italy.
Appl Microbiol Biotechnol. 1996 May;45(4):562-8. doi: 10.1007/BF00578472.
Cells of an aerobic three-membered bacterial co-culture, designated as ECO3, capable of cometabolizing and aerobically dechlorinating low-chlorinated biphenyls in the presence of biphenyl, were immobilized on Manville silica beads, on frosted-glass beads and on polyurethane foam cubes in packed-bed bioreactors continuously fed with a biphenyl-saturated air stream. The ECO3 biofilm reactors were found to be capable of extensively mineralizing several pure dichlorobiphenyls (75 mg/l) and Aroclor 1221 (75 mg/l) in batch mode. Immobilized ECO3 cells could aerobically degrade and dechlorinate the dichlorobiphenyls tested more extensively than suspended ECO3 cells. Among the three biofilm reactors, the glass bead bioreactor and the polyurethane bioreactor exhibited the highest capability of mineralizing both dichlorobiphenyls and Aroclor 1221; the polychlorinated biphenyl availability in the bioreactors more than the biomass availability, both depending on the nature of the support employed, significantly governed the efficiency of the treatment. These results are of interest for the possible development of a bioreactor system for continuous treatment of polychlorinated-biphenyl-contaminated wastewaters.
一种需氧型三元细菌共培养物（命名为ECO3），在联苯存在的情况下能够对低氯代联苯进行共代谢和需氧脱氯，将其固定在曼维尔硅珠、磨砂玻璃珠和聚氨酯泡沫立方体上，并置于填充床生物反应器中，该反应器持续通入联苯饱和气流。研究发现，ECO3生物膜反应器能够在分批模式下将几种纯二氯联苯（75毫克/升）和氯丹1221（75毫克/升）大量矿化。固定化的ECO3细胞比悬浮的ECO3细胞能更广泛地对所测试的二氯联苯进行需氧降解和脱氯。在这三种生物膜反应器中，玻璃珠生物反应器和聚氨酯生物反应器在矿化二氯联苯和氯丹1221方面表现出最高能力；生物反应器中多氯联苯的可利用性超过生物量的可利用性，两者均取决于所用载体的性质，这显著影响了处理效率。这些结果对于开发用于连续处理多氯联苯污染废水的生物反应器系统具有重要意义。
