Brown E J, Resnick S M, Rebstock C, Luong H V, Lindstrom J
Water Research Center, University of Alaska Fairbanks, AK 99775-1760.
Biodegradation. 1991;2(2):121-7. doi: 10.1007/BF00114602.
Following the EXXON Valdez oil spill, a radiorespirometric protocol was developed at the University of Alaska Fairbanks (UAF) to assess the potential for microorganisms in coastal waters and sediments to degrade hydrocarbons. The use of bioremediation to assist in oil spill cleanup operations required microbial bioassays to establish that addition of nitrogen and phosphorus would enhance biodegradation. A technique assessing 1-14C-n-hexadecane mineralization in seawater or nutrient rich sediment suspensions was used for both of these measurements. Hydrocarbon-degradation potentials were determined by measuring mineralization associated with sediment microorganisms in sediment suspended in sterilized seawater and/or marine Bushnell-Haas broth. Production of 14CO2 and CO2 was easily detectable during the first 48 hours with added hexadecane levels ranging from 10 to 500 mg/l of suspension and dependent on the biomass of hydrocarbon degraders, the hydrocarbon-oxidation potential of the biomass and nutrient availability. In addition to assessment of the hydrocarbon-degrading potential of environmental samples, the radiorespirometric procedure, and concomitant measurement of microbial biomass, has utility as an indicator of hydrocarbon contamination of soils, aqueous sediments and water, and can also be used to evaluate the effectiveness of bioremediation treatments.
埃克森·瓦尔迪兹号油轮漏油事件后，阿拉斯加大学费尔班克斯分校（UAF）开发了一种放射性呼吸测定法，以评估沿海水域和沉积物中的微生物降解碳氢化合物的潜力。利用生物修复协助溢油清理作业需要进行微生物生物测定，以确定添加氮和磷是否会增强生物降解作用。一种评估海水中或营养丰富的沉积物悬浮液中1-14C-正十六烷矿化作用的技术被用于这两项测量。通过测量悬浮在无菌海水中和/或海洋布氏肉汤中的沉积物中与沉积物微生物相关的矿化作用，来确定碳氢化合物降解潜力。在最初的48小时内，添加的十六烷水平在每升悬浮液10至500毫克之间时，很容易检测到14CO2和CO2的产生，这取决于碳氢化合物降解菌的生物量、生物量的碳氢化合物氧化潜力和养分有效性。除了评估环境样品的碳氢化合物降解潜力外，放射性呼吸测定程序以及微生物生物量的同步测量，可用作土壤、水相沉积物和水的碳氢化合物污染指标，还可用于评估生物修复处理的有效性。
