一种用于检测产气荚膜梭菌肠毒素的高灵敏度酶联免疫吸附测定法。
A highly sensitive enzyme-linked immunosorbent assay for Clostridium perfringens enterotoxin.
作者信息
Uemura T, Yoshitake S, Hu D, Kajikawa T
机构信息
Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Japan.
出版信息
Lett Appl Microbiol. 1992 Jul;15(1):23-5. doi: 10.1111/j.1472-765x.1992.tb00714.x.
A highly sensitive enzyme-linked immunosorbent assay (ELISA) for quantitation of Clostridium perfringens enterotoxin (CPE) by a sandwich method with polystyrene beads was elaborated. The ELISA was very sensitive with a detection limit of 1 pg/ml of CPE. Clostridium perfringens culture fluid did not interfere with the assay. This ELISA may be useful for the mass screening for Cl. perfringens producing small amounts of CPE.
一种用于通过聚苯乙烯珠夹心法定量产气荚膜梭菌肠毒素（CPE）的高灵敏度酶联免疫吸附测定（ELISA）方法被精心设计出来。该ELISA非常灵敏，CPE的检测限为1 pg/ml。产气荚膜梭菌培养液不干扰该测定。这种ELISA可能有助于对产生少量CPE的产气荚膜梭菌进行大规模筛查。
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