产气荚膜梭菌芽孢上暴露的肠毒素的流式细胞术分析。

Flow cytometric analysis for enterotoxin exposed on Clostridium perfringens spores.

作者信息

Kusunoki H, Hu D, Piyankarage R H, Sugii S, Uemura T

机构信息

Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Japan.

出版信息

J Vet Med Sci. 1998 Dec;60(12):1357-9. doi: 10.1292/jvms.60.1357.

DOI:10.1292/jvms.60.1357

PMID:9879540

Abstract

Flow cytometric method (FCM) with fluorescent-labeled anti-CPE antibody was applied to develop a rapid, specific, and convenient method to detect enterotoxin (CPE) exposed on the surface of spores of Clostridium perfringens. The results obtained indicate that FCM can specifically detect CPE exposed on C. perfringens spores for a short time. Thus, FCM is found to be a rapid, specific, and convenient assay method for detection of CPE exposed on C. perfringens spores, suggesting that it will be hopefully useful to diagnose food poisoning caused by C. perfringens.

摘要

采用荧光标记抗产毒艰难梭菌抗体的流式细胞术（FCM）开发一种快速、特异且便捷的方法，用于检测产气荚膜梭菌芽孢表面暴露的肠毒素（CPE）。所得结果表明，FCM可在短时间内特异性检测产气荚膜梭菌芽孢表面暴露的CPE。因此，发现FCM是一种检测产气荚膜梭菌芽孢表面暴露CPE的快速、特异且便捷的检测方法，这表明它有望用于诊断由产气荚膜梭菌引起的食物中毒。