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用于检测A型产气荚膜梭菌肠毒素的玻片乳胶凝集试验的开发与初步评估

Development and preliminary evaluation of a slide latex agglutination assay for detection of Clostridium perfringens type A enterotoxin.

作者信息

McClane B A, Snyder J T

出版信息

J Immunol Methods. 1987 Jun 26;100(1-2):131-6. doi: 10.1016/0022-1759(87)90181-5.

DOI:10.1016/0022-1759(87)90181-5
PMID:2885377
Abstract

A slide latex agglutination (SLA) assay was developed for rapid screening for Clostridium perfringens type A enterotoxin (CPE). SLA specifically detected CPE added to buffer or normal feces (sensitivity limit of 1 microgram CPE/g feces). Using clinical fecal samples from C. perfringens food poisoning cases, a strong correlation was shown between SLA results and results from other CPE assays and between SLA results and illness status.

摘要

开发了一种玻片乳胶凝集(SLA)试验,用于快速筛查A型产气荚膜梭菌肠毒素(CPE)。SLA能特异性检测添加到缓冲液或正常粪便中的CPE(检测灵敏度为1微克CPE/克粪便)。使用来自产气荚膜梭菌食物中毒病例的临床粪便样本,结果表明SLA结果与其他CPE检测结果之间以及SLA结果与疾病状态之间存在很强的相关性。

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Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test.
采用双重聚合酶链反应和玻片乳胶凝集试验检测食品和粪便样本中的产肠毒素性产气荚膜梭菌。
Appl Environ Microbiol. 1997 Nov;63(11):4232-6. doi: 10.1128/aem.63.11.4232-4236.1997.
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Clostridial enteric diseases of domestic animals.家畜梭菌性肠道疾病
Clin Microbiol Rev. 1996 Apr;9(2):216-34. doi: 10.1128/CMR.9.2.216.
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