ARONSON J M, PRESTON R D
J Biophys Biochem Cytol. 1960 Sep;8(1):247-56. doi: 10.1083/jcb.8.1.247.
Cell walls of the fungus, Allomyces, were isolated by chemical procedures, using either potassium permanganate oxidation or glacial acetic acid-hydrogen peroxide treatment followed by dilute mineral acid. The structure of the treated walls was investigated by means of electron microscopy and electron diffraction analysis which showed that rhizoidal walls were especially suitable for observation. Chitin microfibrils exist in the extreme tips of rhizoidal walls, and tend to lie in a preferred longitudinal orientation. Older rhizoidal wall segments show a crossed fibrillar structure under a thin layer of short randomly arranged microfibrils. In the possession of systems of crossed fibrils these walls are like the cell walls of certain green algae. Walls of branch rhizoidal filaments were observed in the early stages of development, in which case the observed microfibrillar orientations are such that it is possible to envisage their origin from pre-existing fibrils that have passively reoriented. With respect to the continued growth of the filaments, however, it is difficult to explain the observed microfibrillar arrangements in terms of the "multi-net" theory. Hyphal walls usually show two layers, the outer consisting of microfibrils arranged randomly, and the inner consisting of well oriented microfibrils running parallel with the longitudinal axis of the hypha. The oriented inner layer appears to be similar in structure to the secondary wall of the Phycomyces sporangiophore.
真菌异水霉（Allomyces）的细胞壁采用化学方法分离，使用高锰酸钾氧化法或冰醋酸 - 过氧化氢处理，随后用稀无机酸处理。通过电子显微镜和电子衍射分析研究了处理后细胞壁的结构，结果表明，根状细胞壁特别适合观察。几丁质微纤丝存在于根状细胞壁的最顶端，并且倾向于沿优选的纵向方向排列。较老的根状细胞壁段在一层短的随机排列的微纤丝下呈现交叉纤维状结构。这些细胞壁拥有交叉纤维系统，类似于某些绿藻的细胞壁。在分支根状丝的发育早期观察到其细胞壁，在这种情况下，观察到的微纤丝取向使得可以设想它们起源于已被动重新定向的预先存在的纤丝。然而，就丝的持续生长而言，很难用“多网”理论来解释观察到的微纤丝排列。菌丝壁通常显示两层，外层由随机排列的微纤丝组成，内层由与菌丝纵向轴平行排列的取向良好的微纤丝组成。取向的内层在结构上似乎与毛霉目（Phycomyces）孢子囊梗的次生壁相似。
